AEDG is one of those subjects where the details matter more than the headlines. This page pulls together the background, the mechanisms, and the practical points readers ask about most.
Updated 2025-12-19. Numbers and descriptions here follow the published literature rather than marketing material.
Material supplied for laboratory use is normally a lyophilised white to off-white powder that dissolves readily in water and in isotonic saline. Lyophilised cakes are hygroscopic and should be equilibrated to room temperature before opening to limit condensation on the solid. Solutions are typically prepared at milligram-per-millilitre concentrations and divided into single-use aliquots, because repeated freeze–thaw cycles degrade short peptides. Aqueous solutions are far less stable than the dry powder, and identity is usually verified by mass spectrometry alongside purity estimation from reversed-phase high-performance liquid chromatography.
Epitalon is a synthetic linear tetrapeptide with the sequence alanine–glutamate–aspartate–glycine, abbreviated AEDG. Its molecular formula is C14H22N4O9 and the calculated mass is approximately 390.35 g/mol. The compound is made by solid-phase peptide synthesis rather than extracted from tissue, although early work described it as a short fragment of a peptide fraction obtained from bovine pineal extract. In the research literature the spelling epitalon and the variant epithalone both appear, while AEDG is the standard code used in peptide nomenclature.
Several names circulate for the same molecule, including epitalon, epithalone, epithalamin tetrapeptide, and the sequence code AEDG. A CAS registry number, 307297-39-8, is commonly cited for it, though catalogue entries should be checked against supplier documentation because mislabelled records occur. In its usual form the peptide carries free amino and carboxyl termini and is neither glycosylated nor lipidated. Researchers distinguish the defined tetrapeptide from epithalamin itself, a crude pineal preparation containing many peptides that is not chemically characterised.
In its supplied form epitalon is a white to off-white powder, usually lyophilized and often hygroscopic. It dissolves readily in water and in aqueous buffers, and it is commonly handled as the acetate or trifluoroacetate salt to improve stability during purification and drying. The amide backbone is labile in aqueous media, so solutions are less durable than the dry solid. Handling notes in the chemical literature therefore emphasize keeping the powder dry, cool and shielded from light until it is dissolved.
Epitalon is a synthetic tetrapeptide whose sequence is alanine-glutamate-aspartate-glycine, written in single-letter code as AEDG. The four residues are joined by three peptide bonds, giving a linear backbone with no branching and no disulfide bridges. Its calculated molecular mass for the free form is approximately 390.3 daltons, a figure that rises when the compound is supplied as an acetate or trifluoroacetate salt. Because the chain is short, the molecule is defined entirely by its residue order rather than by any folded three-dimensional structure.
| Property | Value | Notes |
|---|---|---|
| Amino acid sequence | Ala-Glu-Asp-Gly | Linear tetrapeptide, also written AEDG |
| Molecular mass | About 390.35 g/mol | Value calculated from the sequence |
| Appearance | White to off-white powder | Lyophilised material forms a loose cake |
| Solubility | Freely soluble in water | Also dissolves in isotonic saline |
| Storage | -20 °C, desiccated | Aliquot solutions to avoid freeze–thaw cycling |
The compound is generally described as a synthetic analogue of a fragment isolated from a pineal gland extract. Researchers associated with the Saint Petersburg Institute of Bioregulation and Gerontology introduced it during the 1980s and 1990s while studying short peptides from animal tissue. The original extract, called epithalamin, is a heterogeneous mixture, whereas epitalon is a single defined sequence. That distinction matters because findings reported for the extract are not automatically findings about the pure tetrapeptide, and claims about broader biological effects remain a separate question from the chemical identity described here.
Residue composition is the property that most cleanly separates verified material from mislabelled samples. Alanine, glutamate, aspartate and glycine appear in that order from the N-terminus, and the two acidic residues sit in the middle of the chain. Because the peptide is short, it can be produced by solid-phase synthesis and characterised by mass spectrometry without ambiguity. Any reported sample whose measured mass departs substantially from 390 daltons is a different compound or a degraded mixture rather than epitalon.
Epitalon is a synthetic tetrapeptide whose four residues are alanine, glutamate, aspartate and glycine, commonly abbreviated AEDG. Its molecular formula is C14H22N4O9 and its monoisotopic mass is near 390.35 daltons. The peptide carries two acidic side chains, so it is neutral to negatively charged in most aqueous buffers. Published reference summaries usually list it under both spellings, epitalon and epithalon, and treat the two names as the same material.
Epitalon is a synthetic tetrapeptide whose sequence is alanine–glutamate–aspartate–glycine, commonly abbreviated AEDG. It was developed in Russia during the 1980s and 1990s by investigators associated with the St. Petersburg Institute of Bioregulation and Gerontology, who studied short peptides as regulators of gene expression and tissue function. The alternative spelling epithalon appears interchangeably in the literature, and the two names refer to the same molecule. Outside Russia it is encountered chiefly as a research chemical rather than a licensed medicine, and it holds no approval from the United States Food and Drug Administration or the European Medicines Agency.
The peptide is a synthetic analogue of epithalamin, a preparation extracted from bovine pineal glands. Investigators sought a short, chemically defined molecule that would reproduce some of the endocrine and gerontological observations attributed to the glandular extract. Proposed mechanisms centre on induction of telomerase activity, an effect reported in cultured human somatic cells in the early 2000s, together with influences on melatonin secretion and neuroendocrine regulation. Those mechanisms remain incompletely characterised, and the reported telomerase response has not been consistently reproduced by independent groups working in comparable systems.
Under the Military Law of September 1925, the Soviet Armed Forces consisted of the Land Forces, the Red Army Air Force, the Navy, Joint State Political Directorate (OGPU) and the Internal Troops. The OGPU later became independent and in 1934 joined the NKVD secret police, and so its internal troops were under the joint leadership of the defence and internal commissariats. After World War II, Strategic Missile Forces (1959), Air Defense Forces (1948) and National Civil Defense Forces (1970) were formed, which ranked first, third, and sixth in the official Soviet system of importance (ground forces were second, Air Force fourth, and Navy fifth). The army had the greatest political influence. In 1989, there served two million soldiers divided between 150 motorized and 52 tank divisions. Until the early 1960s, the Soviet navy was a rather small military branch, but after the Cuban Missile Crisis, under the leadership of Sergei Gorshkov, it expanded significantly. It became known for its submarine fleet. In 1989, there served 500,000 men. The Soviet Air Force focused on a fleet of strategic bombers and during war situation was to eradicate enemy infrastructure and nuclear capacity. The air force also had a number of fighters and tactical bombers to support the army in the war. Strategic missile forces had more than 1,400 intercontinental ballistic missiles (ICBMs), deployed between 28 bases and 300 command centres. After 1945, the Soviet Ground Forces suppressed several uprisings in East Europe and was involved in many other operations abroad.
Official website Mind Changers, Series 4: Arden House BBC Radio programme which interviews Langer about one of her experiments. The Young Ones Ellen Langer's Counter Clockwise study was the basis for this BBC documentary series. The Great Lesson The Great Lesson: A New Film About Mind and Body: Featuring Dr. Ellen Langer
== Treatment == Some of the most common treatments of valvular heart disease are avoiding smoking and excessive alcohol consumption, antibiotics, antithrombotic medications such as aspirin, anticoagulants, balloon dilation, and water pills. In some cases, surgery may be necessary.
== Bioequivalence == In determining bioequivalence between two products such as a commercially available Branded product and a potential to-be-marketed Generic product, pharmacokinetic studies are conducted whereby each of the preparations are administered in a cross-over study (sometimes parallel study, when a cross-over study is not feasible) to volunteer subjects, generally healthy individuals but occasionally in patients. Serum/plasma samples are obtained at prescribed times and assayed for parent drug (or occasionally metabolite) concentration. Occasionally, blood concentration levels are neither feasible or possible to compare the two products (e.g. inhaled corticosteroids), then pharmacodynamic endpoints rather than pharmacokinetic endpoints (see below) are used for comparison. For a pharmacokinetic comparison, the plasma concentration data are used to assess key pharmacokinetic parameters such as area under the curve (AUC), peak concentration (Cmax), time to peak concentration (tmax), and absorption lag time (tlag). Testing should be conducted at several different doses, especially when the drug displays non-linear pharmacokinetics. In addition to data from bioequivalence studies, other data may need to be submitted to meet regulatory requirements for bioequivalence. Such evidence may include:
== Research interests == Among his many research interests, Hunt investigates how the immune system uses peptides to kill diseased cells, and how modifications to chromatin-associated proteins called histones create a "Code" that may be involved in many gene regulation events.
Sources: en.wikipedia.org
=== Global security program === The Lab's work in global security aims to reduce and mitigate the dangers posed by the spread or use of weapons of mass destruction and by threats to energy and environmental security. Livermore has been working on global security and homeland security for decades, predating both the collapse of the Soviet Union in 1991 and the September 11, 2001, terrorist attacks. LLNL staff have been heavily involved in the cooperative nonproliferation programs with Russia to secure at-risk weapons materials and assist former weapons workers in developing peaceful applications and self-sustaining job opportunities for their expertise and technologies. In the mid-1990s, Lab scientists began devising improved biodetection capabilities, leading to miniaturized and autonomous instruments that can detect biothreat agents in a few minutes instead of the days to weeks previously required for DNA analysis. Livermore researchers address a spectrum of threats – radiological/nuclear, chemical, biological, explosives, and cyber. They combine physical and life sciences, engineering, computations, and analysis to develop technologies that solve real-world problems. Activities are grouped into five programs:
Glycogenolysis and gluconeogenesis in liver. Glycogenolysis and lactate release in skeletal muscle. Contract sphincters of Gastrointestinal tract. Thickened secretions from salivary glands. Insulin and glucagon secretion from pancreas. Inhibit histamine-release from mast cells. Increase protein content of secretions from lacrimal glands. Receptor also present in cerebellum. Bronchiole dilation (targeted while treating asthma attacks) Involved in brain - immune - communication Short-acting β2 agonists (SABA) bitolterol fenoterol hexoprenaline isoprenaline (INN) or isoproterenol (USAN) levosalbutamol (INN) or levalbuterol (USAN) orciprenaline (INN) or metaproterenol (USAN) pirbuterol procaterol salbutamol (INN) or albuterol (USAN) terbutaline Long-acting β2 agonists (LABA) arformoterol (some consider it to be an ultra-LABA) bambuterol clenbuterol formoterol salmeterol Ultra-long-acting β2 agonists (ultra-LABA) carmoterol indacaterol milveterol (GSK 159797) olodaterol vilanterol (GSK 642444)
Eq. 2 where: 3 is the number of fatty acids residues per triglyceride 1000 is the conversion factor for milligrams to grams 56.1 is the molar mass of KOH. 38.049 is the molecular mass of glycerol backbone For instance, triolein, a triglyceride occurring in many fats and oils, has three oleic acid residues esterified to a molecule of glycerol with a total MW of 885.4 (g / mol). Therefore, its SV equals 190 mg KOH / g sample. In comparison, trilaurin with three shorter fatty acid residues (lauric acid) has a MW of 639 and an SV of 263. As it can be seen from equation (2), the SV of a given fat is inversely proportional to its molecular weight. Actually, as fats and oils contain a mix of different triglycerides species, the average MW can be calculated according to the following relation:
Another form of bioprinting involves an inkjet printer, which is primarily used in biomedical settings. This method prints detailed proteins and nucleic acids. Hydrogels are commonly selected as the bioink. Cells can be printed on to a selected surface media to proliferate and ultimately differentiate. A drawback of this printing method is the ability of the bioinks such as hydrogels to clog the printing nozzle, due to their high viscosity. Ideal inkjet bioprinting involves using a low polymer viscosity (ideally below 10 centipoise), low cell density (<10 million cells/mL), and low structural heights (<10 million cells/mL).
Sources: en.wikipedia.org
=== Pretreatment === Pretreatment includes any method of treating the product prior to freezing. This may include concentrating the product, formulation revision (i.e., addition of components to increase stability, preserve appearance, and/or improve processing), decreasing a high-vapor-pressure solvent, or increasing the surface area. Food pieces are often IQF treated to make them free flowing prior to freeze drying. Freeze dried pharmaceutical products are in most cases parenterals administered after reconstitution by injection which need to be sterile as well as free of impurity particles. Pre-treatment in these cases consists of solution preparation followed by a multi-step filtration. Afterwards the liquid is filled under sterile conditions into the final containers which in production scale freeze dryers are loaded automatically to the shelves. In many instances the decision to pretreat a product is based on theoretical knowledge of freeze-drying and its requirements, or is demanded by cycle time or product quality considerations.
Acetyl-CoA is generated in the mitochondria from pyruvate molecules derived from glucose via glycolysis. Within the mitochondria, acetyl-CoA typically combines with oxaloacetate and serves as a substrate for the synthesis of citrate as part of the well known citric acid cycle. Notably, the inner mitochondrial membrane is impermeable to acetyl-CoA, and as such, a specialized shuttle system must be used to import acetyl-CoA into the cytosol for fatty acid production. This process, known as the citrate–malate shuttle, relies on the tricarboxylate transport protein to import citrate into the cytosol, where it is then split into acetyl-CoA and oxaloacetate by the enzyme ATP citrate lyase (ACL). Cytosolic acetyl-CoA is then available for use in fatty acid and cholesterol synthesis, but oxaloacetate must be reduced to malate in order to reenter the mitochondria. Malate dehydrogenase reduces cytosolic oxaloacetate by coupling NADH oxidation to NAD+, and malate produced by this reaction can be transported back into the mitochondria, thus completing the namesake of the citrate–malate shuttle.
== Further reading == Bolsmann, Chris (1 November 2021). "'Playing With Apartheid': Irish and South African Rugby, 1964–19891". Sport History Review. 52 (2): 262–278. doi:10.1123/shr.2020-0027. S2CID 235043351. Booth, Douglas (1 July 2003). "Hitting Apartheid for Six? The Politics of the South African Sports Boycott". Journal of Contemporary History. 38 (3): 477–493. doi:10.1177/0022009403038003008. S2CID 145730533. Booth, Douglas (2013). "Recapturing the Moment? Global Rugby, Economics and the Politics of Nation in Post-Apartheid South Africa". In Chandler, Timothy J.L.; Nauright, John (eds.). Making the Rugby World. pp. 181–200. doi:10.4324/9781315036984. ISBN 978-1-135-22722-7. Nixon, Rob (1992). "Apartheid on the Run: The South African Sports Boycott". Transition (58): 68–88. doi:10.2307/2934968. JSTOR 2934968. Snyders, Hendrik (3 July 2018). "'An Outrage, Not Athletics': Apartheid and South African–United States Rugby Relations, 1976–1990". The International Journal of the History of Sport. 35 (10): 1029–1059. doi:10.1080/09523367.2019.1576636. S2CID 150831234. Snyders, Hendrik (2022). "Rugby, reconciliation, and post-apartheid public memory". Sport in Museums. pp. 80–91. doi:10.4324/9781351117944-6. ISBN 978-1-351-11794-4.
Sources: en.wikipedia.org
It is a synthetic tetrapeptide. Its sequence was derived from research on peptide fractions of bovine pineal extract, and the intact AEDG sequence has not been demonstrated as an abundant free peptide in human tissue.
AEDG is the one-letter code for the four amino acids alanine, glutamate, aspartate and glycine in that order. The same molecule is written in three-letter form as Ala-Glu-Asp-Gly.
Identity is normally confirmed by mass spectrometry against the calculated mass of roughly 390.35 g/mol. Purity is then estimated by reversed-phase high-performance liquid chromatography with ultraviolet detection.
The peptide contains four residues in the order alanine, glutamate, aspartate and glycine, abbreviated AEDG. The chain is linear and held together by three peptide bonds. Its calculated mass for the uncharged free form is about 390.3 daltons.